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目的检测脂多糖(Lipopolysaccharides,LPS)诱导THP-1巨噬细胞固有免疫反应不同时间点(4、8、12、24、48 h)差异表达的microRNAs(miRNAs)及预测的靶基因的表达情况,探讨骨修复材料移植早期的宿主免疫炎症反应调控机制。方法 LPS处理由佛波醇PMA诱导分化的THP-1细胞,分别在刺激4、8、12、24、48 h之后提取总RNA,用实时荧光定量PCR方法检测microRNAs表达。生物信息学预测miR-10b靶基因是SCARB2并检测其蛋白表达。结果 miR-10b、let-7家族以及miR-181c与芯片结果相符。SCARB2预测为miR-10b的靶基因,且蛋白表达上升。结论 miR-10b可能通过靶基因SCARB2参与调控炎症免疫过程。
Objective To detect the expression of microRNAs (miRNAs) and target genes predicted by Lipopolysaccharides (LPS) -induced THP-1 macrophage innate immune responses at different time points (4,8,12,24,48 h) To investigate the mechanism of host immune-inflammatory response in the early stage of bone repair material transplantation. Methods THP-1 cells induced by phorbol-PMA were treated with LPS. Total RNA was extracted after stimulation for 4, 8, 12, 24, and 48 h, respectively. The expression of microRNAs was detected by real-time fluorescence quantitative PCR. Bioinformatics predicts that the miR-10b target gene is SCARB2 and detects its protein expression. Results The miR-10b, let-7 family and miR-181c were in good agreement with the results from the chip. SCARB2 is predicted to be the target gene of miR-10b, and the protein expression is increased. Conclusion miR-10b may be involved in the regulation of inflammatory and immune processes through its target gene SCARB2.