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目的:研究体外培育牛黄(CBS)对葡聚糖硫酸钠(DSS)诱导的慢性溃疡性结肠炎(UC)的治疗作用。方法:采用2%的DSS溶液连续给药3周期,在雄性C57BL/6小鼠中诱导慢性UC动物模型。造模的最后7 d连续给予50 mg·kg~(-1)和150mg·kg~(-1)的CBS灌胃给药处理,每日1次;通过小鼠一般情况、疾病活动(DAI)指数、结肠状态、HE染色和Tunel检测细胞凋亡等来评价CBS对DSS诱导慢性UC的疗效。结果:CBS减弱了DSS诱导的小鼠体质量降低、腹泻、便血等症状,并减少了其DAI指数,同时减轻了结肠的组织病理损伤。结论:CBS对于DSS诱导的慢性UC具有一定的治疗作用,值得进一步深入研究。
AIM: To investigate the therapeutic effect of bezoar (CBS) in vitro on dextran sodium sulfate (DSS) -induced chronic ulcerative colitis (UC). Methods: Chronic UC animal model was induced in male C57BL / 6 mice by continuous administration of 2% DSS solution for 3 cycles. In the last 7 days of modeling, CBS administered 50 mg · kg -1 and 150 mg · kg -1 were administered intragastrically once daily. Index, colon status, HE staining and Tunel apoptosis detection to evaluate the effect of CBS on DSS-induced chronic UC. Results: CBS attenuated DSS-induced symptoms such as decreased body weight, diarrhea, blood in the stool and decreased its DAI index, meanwhile, it reduced histopathological damage in colon. Conclusion: CBS has certain therapeutic effect on chronic UC induced by DSS, which deserves further study.