利用细胞松弛素B改进淋巴细胞微核检测方法

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微核测试技术已广泛作为检测染色体损伤的方法.在微核检测中,尤其是体外染毒时,只有观察至少经过一次分裂的细胞才有意义,因为通过一次细胞分裂,染色体损伤才能以微核的形式表达出来.由于供血者的个体差异和培养条件等的不同,淋巴细胞培养时,各细胞的动力学过程有很大差别,即培养期间细胞分裂过程并不同步,有的可不分裂,有的分裂过1次、2次或更多次,而且常规 Micronucleus test technology has been widely used as a method of detecting chromosomal damage. In micronucleus test, especially in vitro exposure, only the observation of at least one division of cells makes sense, because by a cell division, chromosome damage to micronuclei Of the form of expression.Due to individual differences in blood donors and culture conditions, lymphocyte culture, the kinetic process of each cell is very different, that is, cell division during culture is not synchronized, and some can not divide, there are Split 1, 2 or more times, and routine
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