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目的:探讨心理应激诱导的肠易激综合征(IBS)大鼠胃肠激素的变化及宁肠汤的调节作用。方法:将72只雄性大鼠随机分为正常对照组、模型对照组、得舒特组、宁肠汤低、中、高剂量组,以慢性束缚+适度夹尾刺激作为心理应激源诱导IBS模型,观察宁肠汤对IBS模型鼠结肠转运功能,并以酶联免疫分析法检测各组血清CCK的变化。结果:模型组结肠转运功能较正常组明显亢进(P<0.01),而各治疗组较模型组下降(P<0.05);模型组血清CCK水平较正常对照组明显升高(P<0.01),与模型对照组比较,各治疗组血清CCK含量明显降低(P<0.01)。结论:宁肠汤可调节束缚应激致腹泻模型鼠的结肠转运功能,降低已升高的血清CCK含量,且具一定的量效关系。
Objective: To investigate the changes of gastrointestinal hormones induced by psychological stress in irritable bowel syndrome (IBS) rats and the regulation effect of Ningchang Decoction. Methods: Seventy-two male rats were randomly divided into normal control group, model control group, dextrose group and ningchang decoction low, medium and high dose group. Chronic restraint + moderate pinch-tail stimulus was used as psychological stress source to induce IBS Model to observe the intestine transit function of Ningchang Decoction on IBS model rats, and the change of serum CCK in each group was detected by enzyme-linked immunosorbent assay. Results: The colonic transit function of the model group was significantly higher than that of the normal group (P <0.01), but decreased in each treatment group (P <0.05). The serum CCK level in the model group was significantly higher than that in the normal control group (P <0.01) Compared with the model control group, serum CCK levels in each treatment group decreased significantly (P <0.01). CONCLUSION: Ningchang Decoction can regulate colonic transit function of rats with restraint stress induced by restraint stress and reduce the elevated serum CCK content, with a certain dose-effect relationship.