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目的 :建立eNOS基因VNTR多态性的PCR分析方法。方法 :按eNOS基因VNTR位点侧翼序列设计引物 ,自健康献血者基因组DNA扩增VNTR片段 ,琼脂糖凝胶电泳分析扩增片段的长度和数目 ,鉴定VNTR基因型。结果与结论 :在 2 0 8名健康献血者中鉴定出 6/ 5杂合、5 / 5纯合、5 / 4杂合和 4 / 4纯合 4种VNTR基因型 ;等位基因的分布频率与日本人相似 ,但与美国黑人存在显著差异
Objective: To establish a PCR analysis method of eNOS VNTR polymorphism. Methods: Primers were designed according to the flanking sequences of VNTR site of eNOS gene. VNTR fragments were amplified from genomic DNA of healthy donors. The length and number of amplified fragments were analyzed by agarose gel electrophoresis to identify the VNTR genotypes. RESULTS AND CONCLUSION: Four VNTR genotypes of 6/5 heterozygous, 5/5 homozygous, 5/4 heterozygous and 4/4 homozygote were identified among 280 healthy blood donors. The frequency of allele distribution Similar to Japanese, but significantly different from African Americans