目的　研究罗哌卡因对豚鼠心室肌细胞膜离子通道的影响 ,探讨其心脏毒性作用的发生机理。方法　用酶解法获得单个的左心室肌细胞 ,用标准的全细胞膜片钳技术记录用药前后的钠流 (INa)、L 型钙流 (ICa L)、延迟整流性钾流 (IK)和内向整流性钾流 (IK1)的变化。结果　罗哌卡因对INa及ICa L都有抑制作用 ,且呈浓度依赖性。 5 0 μmol L 1罗哌卡因可使INa、ICa L分别下降 36 8%、7 6 8% ;10 0 μmol L 1罗哌卡因可使INa、ICa L分别下降 6 6 1%、2 3 13% ;对INa的抑制作用强于ICa L(P <0 0 5 ) ,10 0 μmol L 1罗哌卡因对IK及IK1无影响 (P >0 0 5 )。结论　罗哌卡因的心脏毒性作用与抑制钠通道和钙通道有关。 Objective To study the effect of ropivacaine on the ion channels of guinea pig ventricular myocytes and to explore its mechanism of cardiotoxicity. Methods Single left ventricular myocytes were obtained by enzymolysis. INa, ICa L, IK and IK were recorded before and after treatment with standard whole-cell patch clamp technique. Changes in potassium (IK1). Results Ropivacaine inhibited both INa and ICa L in a concentration-dependent manner. The levels of INa and ICa L were decreased by 36.8% and 78.6% respectively when 50 μmol L 1 of ropivacaine was added, while the levels of INa and ICa L were decreased by 6 6 1% and 10 3 13%. The inhibitory effect on INa was stronger than that on ICa L (P <0.05). The effect of 10 μmol·L -1 ropivacaine on IK and IK1 was not significant (P> 0.05). Conclusion Cardiotoxicity of ropivacaine is related to inhibition of sodium channel and calcium channel.