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目的:探讨补气消癜汤对免疫性血小板减少症模型(ITP)小鼠的白细胞介素系列因子及肿瘤坏死因子-α(TNF-α)的影响,旨在为临床治疗提供依据。方法:采用注射豚鼠抗小鼠血小板血清(GP-APS)方法建立ITP小鼠,随机将72只小鼠分为6组:空白组、模型组、强的松组、补气消癜汤组(低、中、高剂量),观察造模前、给药前、给药后小鼠外周血血小板计数(PLT);给药后,观察小鼠的脾脏系数、胸腺系数、骨髓巨核细胞数(MN)、脾脏MN,采用ELISA法测定小鼠血清白介素-2(IL-2)、IL-6、IL-10及肿瘤坏死因子-α(TNF-α)、血小板生成素(TPO)含量的变化。结果:1给药前,与空白组对比,模型组、强的松组、补气消癜汤各剂量组的PLT均出现明显下降(P<0.01);给药后,各组小鼠的PLT较造模前减少,较给药前增多,差异显著(P<0.01);与模型组对比,补气消癜汤各剂量组的PLT和TPO明显增多(P<0.01)。2与空白组对比,模型组的脾脏系数、胸腺系数、脾脏MN、骨髓MN均明显增多(P<0.01),与模型组对比,补气消癜汤各剂量组的脾脏系数、胸腺系数、脾脏MN、骨髓MN明显减少(P<0.01或P<0.05),与强的松组对比,补气消癜汤高剂量组的脾脏系数、胸腺系数、脾脏MN明显减少(P<0.05)。3与空白组对比,模型组的IL-2、IL-6及TNF-α升高,IL-10降低,差异显著(P<0.01);与模型组对比,强的松组、补气消癜汤各剂量组的IL-2、IL-6及TNF-α降低,IL-10升高,差异显著(P<0.01);与强的松组对比,补气消癜汤高剂量组的IL-2及TNF-α降低,IL-10升高,差异显著(P<0.01或P<0.05)。结论:补气消癜汤可通过调节ITP小鼠体内IL-2、IL-6、IL-10及TNF-α分泌,促进脾脏和骨髓的巨核细胞分化,增加ITP模型小鼠外周血小板数。
Objective: To investigate the effect of Buqilhexian decoction on interleukin-series factor and tumor necrosis factor-α (TNF-α) in immune-induced thrombocytopenia model (ITP) mice, and to provide basis for clinical treatment. Methods: ITP mice were established by intraperitoneal injection of guinea pig anti-mouse platelet (GP-APS), and 72 mice were randomly divided into 6 groups: blank group, model group, prednisone group, Low, middle and high dose). Before and after administration, the platelet count (PLT) of peripheral blood of mice before and after administration was observed. After administration, the spleen coefficient, thymus index and the number of bone marrow megakaryocytes ), Splenic MN. The levels of serum IL-2, IL-6, IL-10, TNF-α and TPO were measured by ELISA. Compared with the blank group, the PLT of model group, prednisone group and qiqihexingtang decoction group decreased obviously (P <0.01) before administration; 1. After administration, the PLT Compared with the model group, the PLT and TPO in each dose group of Buqi Qigang Decoction increased significantly (P <0.01), compared with the model group. Compared with the blank group, the spleen coefficient, thymus coefficient, splenic MN and myeloid MN in the model group were significantly increased (P <0.01). Compared with the model group, the spleen coefficient, thymus coefficient, MN and bone marrow MN significantly decreased (P <0.01 or P <0.05). Compared with prednisone group, the spleen coefficient, thymus coefficient and splenic MN of high-dose group of Buqiheidang decoction decreased significantly (P <0.05). Compared with the blank control group, the levels of IL-2, IL-6 and TNF-α in the model group increased and IL-10 decreased significantly (P <0.01) The levels of IL-2, IL-6, TNF-α and IL-10 in each dose group were significantly lower than those in the prednisone group (P <0.01) 2, TNF-α and IL-10 were significantly increased (P <0.01 or P <0.05). Conclusion: Buqi Decoction can promote the differentiation of megakaryocytes in spleen and bone marrow and increase the number of peripheral platelets in ITP mice by regulating the secretion of IL-2, IL-6, IL-10 and TNF-α in ITP mice.