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目的 研究激素敏感型肾病综合征 (SSNS)患儿活动期和缓解期血清血管内皮细胞生长因子 (VEGF)水平及临床意义。方法 应用酶联免疫吸附测定 (ELISA)方法检测 2 2例SSNS活动期和缓解期的VEGF水平 ,以 18例年龄、性别匹配的正常儿童作对照 ;同时测定血尿素氮 (BUN)、肌酐 (Cr)和 2 4h尿蛋白定量 ,并分析与VEGF的关系。结果 SSNS患儿活动期血VEGF水平 [(64 3 5± 16 5 9)ng/L]高于缓解期 [(3 5 17± 13 67)ng/L]和正常对照 [(3 3 4± 5 9)ng/L] ,P <0 0 5 ;缓解期与正常对照组相比 ,无明显差异 ,P >0 0 5 ;活动期VEGF水平与BUN、Cr无相关 ,而与 2 4h尿蛋白定量呈正相关。结论 SSNS患儿活动期VEGF水平升高 ,并与蛋白尿呈正相关 ;激素治疗可降低VEGF水平 ,提示VEGF参与SSNS的发病
Objective To study the serum levels of vascular endothelial growth factor (VEGF) and its clinical significance in active and remission children with steroid sensitive nephrotic syndrome (SSNS). Methods Serum levels of VEGF in 22 cases of active and remission of SSNS were detected by enzyme-linked immunosorbent assay (ELISA). 18 cases of age-matched and normal-matched children were used as control. Blood urea nitrogen (BUN), creatinine ) And 24 h proteinuria, and analyze the relationship with VEGF. Results The serum levels of VEGF in active stage of SSNS patients were significantly higher than those in normal controls [(64 3 5 ± 16 5 9) ng / L] [(3 5 17 ± 13 67) ng / L] 9) ng / L], P <0 05. There was no significant difference between the remission period and the normal control group (P> 0.05). The level of VEGF in active stage was not correlated with BUN and Cr, Was positively correlated. Conclusion The activity of VEGF in SSNS children is elevated and positively correlated with proteinuria. Hormone therapy can reduce the level of VEGF, suggesting that VEGF is involved in the pathogenesis of SSNS