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采用聚丙烯酸胺凝胶电泳的方法,对通过外源DNA导入法所获的大豆变异后代,进行过氧化物酶和酯酶的酶谱分析。其结果是酶谱谱带清晰,变异后代与亲本的谱带有明显差异,并与该材料的表型性状相一致。因此,我们认为可以用同工酶分析法做为鉴定外源DNA导入大豆的生化指标之一。
Polyacrylamide gel electrophoresis was used to analyze the zymogram of peroxidase and esterase in soybean variant progeny obtained by the exogenous DNA introduction method. The result was a clear zymogram band, a significant difference in the bands between the mutant offspring and the parent, consistent with the phenotypic traits of the material. Therefore, we think that isozyme assay can be used as one of the biochemical markers for the identification of foreign DNA into soybean.