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目的:探讨采用动态对比增强磁共振成像(dynamic contrast-enhanced magnetic resonance imaging,DCE-MRI)评估125I粒子组织间植入治疗人胰腺癌裸鼠移植瘤疗效的价值。方法:将人胰腺癌SW1990细胞株接种于BABL/c裸鼠右下肢旁腹股沟区偏背侧皮下,当瘤体直径长至8～10 mm时进行干预,共16只成瘤大小合适的裸鼠用于实验,分别植入125I粒子(8只,实验组)和空载粒子(8只,对照组),在粒子植入前及治疗后2周及2个月行MRI常规扫描及DCE-MRI成像,并对肿瘤标本行组织病理学检查。结果:实验组肿瘤细胞坏死明显,而对照组肿瘤细胞无明显或有少量坏死。所有裸鼠的心、肝、肺、肾及脾脏等组织均无明显放射炎症表现。常规MRI评价125I粒子治疗胰腺癌疗效的价值有限。实验组肿瘤实质与坏死交界区的Ktrans值最高,其次为整个肿瘤组织的Ktrans值,再次为肿瘤实质,最后是肿瘤坏死区。实验组治疗后2周及2个月裸鼠的整个肿瘤组织Ktrans值均较治疗前降低,且整个肿瘤组织的Ktrans与肿瘤坏死率有间一定的相关性(r=-0.518,P=0.01);而肿瘤坏死率与肿瘤体积间有一定相关性,坏死随着肿瘤体积增加而增加(r=0.641,P=0.001)。结论:125I粒子治疗人胰腺癌裸鼠可导致移植瘤内坏死,且对周围脏器较安全。用常规MRI及DCE-MRI对裸鼠皮下移植瘤成像是可行的。常规MRI对治疗疗效的评估作用有限,而DCE-MRI则可定量分析125Ⅰ粒子治疗后肿瘤血管灌注特性的改变,对疗效的评估有重要价值。
Objective: To evaluate the value of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) in assessing the therapeutic effect of 125I interstitial implantation in human pancreatic cancer xenografts in nude mice. Methods: Human pancreatic cancer cell line SW1990 was inoculated subcutaneously in the dorsal limb of the right lower extremity in BABL / c nude mice. When the diameter of the tumor reached 8-10 mm, a total of 16 nude mice For the experiment, 125I particles (8 experimental groups) and empty particles (8 control groups) were implanted. Conventional MRI and DCE-MRI before and after 2 and 2 weeks Imaging, and histological examination of tumor specimens. Results: The necrosis of tumor cells in experimental group was obvious, but there was no obvious or slight necrosis of tumor cells in control group. All nude mice heart, liver, lung, kidney and spleen and other organizations showed no significant radiation inflammation. Conventional MRI evaluation of 125I particles in the treatment of pancreatic cancer has limited value. In the experimental group, the Ktrans value of the junction between the parenchyma and the necrotic area was the highest, followed by the Ktrans value of the entire tumor tissue, again the tumor mass and finally the tumor necrosis area. The Ktrans values of the whole tumor tissue of the nude mice in the experimental group were lower than those before treatment at 2 weeks and 2 months after treatment, and there was a certain correlation between Ktrans and tumor necrosis rate (r = -0.518, P = 0.01) The tumor necrosis rate was correlated with the tumor volume. The necrosis increased with tumor volume (r = 0.641, P = 0.001). Conclusion: The treatment of 125I particles in human pancreatic cancer nude mice can lead to necrosis in the transplanted tumor and is safer for the surrounding organs. Imaging of subcutaneously transplanted nude mice with conventional MRI and DCE-MRI is feasible. Conventional MRI is of limited value in the assessment of curative effect, while DCE-MRI can quantitatively analyze the change of tumor perfusion characteristics after 125I particle therapy, which is of great value in the evaluation of curative effect.