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目的研究实时荧光定量聚合酶链式反应与快速培养法在肺炎支原体检验中的应用效果。方法选择2013年3月~2015年3月我院接诊的200例疑似肺炎支原体感染的患者进行研究,采用实时荧光定量聚合酶链式反应与快速培养法对患者的咽拭子进行支原体检测分析。结果快速培养法的检测结果显示:敏感度为83.48%,特异度为75.29%,诊断符合率为诊断符合率为80.00%。实时荧光定量聚合酶链式反应的检测结果显示:敏感度为84.31%,特异度为81.63%,诊断符合率为83.00%。两种方法联合的检测结果显示:敏感度为90.90%,特异度为91.14%,诊断符合率为91.00%。实时荧光定量聚合酶链式反应联合快速培养法诊断的敏感度、特异度及诊断符合率均明显高于两种检测手段的单独检测,差异有统计学意义(P<0.05)。但两种检测手段的结果不完全一致(一致率为76.00%)。结论实时荧光定量聚合酶链式反应与快速培养法联合对肺炎支原体进行检测,可显著提高检测的灵敏度、特异度和诊断符合率,值得广泛推广。
Objective To study the application of real-time fluorescence quantitative polymerase chain reaction and rapid culture in Mycoplasma pneumoniae. Methods A total of 200 patients suspected of Mycoplasma pneumoniae infection from March 2013 to March 2015 in our hospital were selected for mycoplasma infection. Mycoplasma was detected by real-time fluorescence quantitative polymerase chain reaction and rapid culture . Results The results of rapid culture method showed that the sensitivity was 83.48% and the specificity was 75.29%. The diagnostic coincidence rate was 80.00%. The detection results of real-time fluorescence quantitative polymerase chain reaction showed that the sensitivity was 84.31%, the specificity was 81.63%, and the diagnostic coincidence rate was 83.00%. The combined test results of the two methods showed that the sensitivity was 90.90%, the specificity was 91.14%, and the diagnostic coincidence rate was 91.00%. The sensitivity, specificity and diagnostic coincidence rate of real-time PCR and rapid culture were significantly higher than those of the two detection methods, the difference was statistically significant (P <0.05). However, the results of the two tests are not exactly the same (consistency rate of 76.00%). Conclusion The detection of mycoplasma pneumoniae by real-time fluorescence quantitative polymerase chain reaction combined with rapid culture method can significantly improve the sensitivity, specificity and diagnostic coincidence rate of detection, which is worth popularizing widely.