Objective: To investigate the anti-inflammatory activity of different fractions and glutinol(isolated compound),using nitric oxide synthase and cyclooxygenase(COX) inhibition as an indication of anti-inflammatory activity,Methods: Anti-inflammatory activity was evaluated using an in vitro assay determining the inhibition of the activity of pro-inflammatory enzyme model,Cyclooxygenases and inducible nitric oxide synthase are crucial enzymes involved in the pathogenesis of many chronic inflammatory conditions,Results: Sub-fraction F3.3 that was derived from n-hexane fraction of PA leaves significantly inhibited(P = 0.01) the catalytic activity of COX-2(IC50 = 0.67 μg/m L) better than isolated compound,glutinol(IC50 = 1.22 μg/m L),compound 2(CP2)(IC50 = 1.71 μg/m L) and sub-fraction F3.3.0(IC50 = 1.30 μg/m L),A similar trend was observed in investigation of the inhibition of nitric oxide synthesis in RAW 264.7 cells by F3.3,glutinol,CP2 and F3.3.0,Inducible COX-2 and inducible nitric oxide synthase are among potent signalling enzymes that exacerbate inflammation,Conclusions: Bioactive sub-fractions(F3.3 and F3.3.0) derived from the n-hexane fraction of PA had good anti-inflammatory activity,and the isolated compound,and glutinol may be useful as a template for the development of new anti-inflammatory drugs. Objective: To investigate the anti-inflammatory activity of different fractions and glutinol (isolated compound), using nitric oxide synthase and cyclooxygenase (COX) inhibition as an indication of anti-inflammatory activity, Methods: Anti-inflammatory activity was evaluated using an in vitro assay determining the inhibition of the activity of pro-inflammatory enzyme model, Cyclooxygenases and inducible nitric oxide synthase are crucial enzymes involved in the pathogenesis of many chronic inflammatory conditions, Results: Sub-fraction F3.3 that was derived from n-hexane fraction of PA leaves significantly inhibited (P = 0.01) the catalytic activity of COX-2 (IC50 = 0.67 μg / m L) better than isolated compound, glutinol IC50 = 1.22 μg / m L) μg / mL) and sub-fraction F3.3.0 (IC50 = 1.30 μg / mL), A similar trend was observed in the investigation of the inhibition of nitric oxide synthesis in RAW 264.7 cells by F3.3, glutinol, CP2 and F3 .3.0, Inducible COX-2 and inducible nitric oxide synthase are among potent signaling enzymes that exacerbate inflammation, Conclusions: Bioactive sub-fractions (F3.3 and F3.3.0) derived from the n-hexane fraction of PA had good anti-inflammatory activity, and the isolated compound, and glutinol may be useful as a template for the development of new anti-inflammatory drugs.