论文部分内容阅读
目的:探讨载羟基喜树碱聚乳酸微泡超声辐照下对肝癌细胞的生物学效应。方法:选择人肝癌细胞株Bel-7402为体外模型,采用MTT比色分析法,观察羟基喜树碱聚乳酸微泡对肝癌细胞的生长和增殖抑制情况,评价载药微泡对肝癌细胞的毒性作用;采用激光共聚焦荧光显微镜技术和流式细胞仪技术观察超声辐照下载药微泡对肝癌细胞摄取药物的影响。结果:羟基喜树碱聚乳酸微泡抑制肝癌细胞的生长增殖,且随羟基喜树碱浓度的升高而增强;激光共聚焦荧光照片显示:与未超声的肝癌细胞相比,超声辐照下,肝癌细胞内羟基喜树碱的绿色荧光增强,说明细胞摄取羟基喜树碱的量增加;流式细胞仪数值:细胞内羟基喜树碱荧光值对照组仅37.5,未超声实验组为71.9;超声实验组为91.3。结论:羟基喜树碱聚乳酸微泡对肝癌细胞Bel-7402有毒性作用,且超声辐照对细胞摄取药物有促进作用。其超声辐照下对肿瘤细胞生物学效应的评价可将为其今后的临床超声治疗提供必要依据。
Objective: To investigate the biological effects of hydroxycamptothecin-loaded polylactic acid microbubbles on hepatoma cells under ultrasound irradiation. METHODS: Human hepatoma cell line Bel-7402 was selected as an in vitro model. MTT colorimetric assay was used to observe the growth and proliferation inhibition of HCPT microbubbles on hepatocellular carcinoma cells, and evaluate the toxicity of drug-loaded microbubbles on hepatocellular carcinoma cells. Effects; The effects of ultrasound-loaded drug-loaded microbubbles on the uptake of drugs by hepatoma cells were observed by laser confocal fluorescence microscopy and flow cytometry. RESULTS: Hydroxycamptothecin polylactic acid microbubbles inhibited the growth and proliferation of hepatocellular carcinoma cells and increased with the increase of hydroxycamptothecin concentration. Confocal laser fluorescence photographs showed that compared with non-ultrasonic hepatocellular carcinoma cells, ultrasound irradiation The increase of the green fluorescence of hydroxycamptothecin in hepatocellular carcinoma cells indicates that the amount of hydroxycamptothecin is taken up; the flow cytometry value: the intracellular hydroxycamptothecin fluorescence value of the control group is only 37.5, and the non-ultrasound group is 71.9; The ultrasonic test group was 91.3. CONCLUSION: Hydroxycamptothecine-based polylactic acid microbubbles have toxic effects on hepatoma cells Bel-7402, and ultrasound irradiation can promote the uptake of drugs by the cells. The evaluation of the biological effects of tumor cells under ultrasound irradiation can provide the necessary basis for future clinical ultrasound therapy.