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目的:探讨喉鳞状细胞癌组织中微血管密度(MVD)和血管内皮生长因子(VEGF)、碱性成纤维生长因子(bFGF)的表达及其临床意义。方法:采用免疫组织化学SP法对42例喉鳞状细胞癌组织及10例癌旁正常组织常规石蜡切片染色,分别检测VEGF、bFGF和CD31。结果:①在喉鳞状细胞癌中,VEGF、bFGF主要分布在肿瘤细胞的胞质内,其阳性率分别为69.04%(29/42)、61.9%(26/42)。②VEGF、bFGF表达与TNM分期、颈淋巴结转移有关(P<0.05)。③声门上型组、颈淋巴结转移组、中、晚期喉癌组组织内的MVD明显高于声门型组、无颈淋巴结转移组及早期喉癌组(P<0.05)。结论:VEGF和bFGF在喉癌的血管生成中起着重要作用,二者与肿瘤的浸润、转移和复发密切相关。
Objective: To investigate the expression of microvessel density (MVD), vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in laryngeal squamous cell carcinoma and its clinical significance. Methods: Immunohistochemical SP method was used to stain the paraffin sections of 42 cases of laryngeal squamous cell carcinoma and 10 cases of adjacent normal tissues. VEGF, bFGF and CD31 were detected respectively. Results: ① In laryngeal squamous cell carcinoma, VEGF and bFGF mainly distributed in the cytoplasm of tumor cells, the positive rates were 69.04% (29/42) and 61.9% (26/42), respectively. ② The expression of VEGF and bFGF was correlated with TNM stage and cervical lymph node metastasis (P <0.05). ③ The MVD in supraglottic, cervical lymph node metastasis, middle and late laryngeal carcinoma was significantly higher than that in glioma, cervical lymph node metastasis and early laryngeal carcinoma (P <0.05). Conclusion: VEGF and bFGF play an important role in the angiogenesis of laryngeal carcinoma, which are closely related to the infiltration, metastasis and recurrence of the tumor.