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目的:探讨扶正祛邪双表法对流感病毒感染致病毒性肺炎小鼠的治疗作用。方法:以流感病毒A/FM/47(H1N1)鼠肺适应株滴鼻感染昆明种小鼠,并用解表药(银翘散)、固表药(玉屏风散)、双表药(玉屏风散+银翘散),银屏散、阳性对照药(利巴韦林)干预相应组别小鼠。在流感病毒感染后3、5、7 d,计算肺指数和抑制率,观察肺组织切片光镜下的病理改变;采用实时荧光定量PCR(real time RT-PCR)检测小鼠肺组织中病毒载量的变化。结果:银翘散、玉屏风散、银屏散在感染后第3、5、7 d可明显降低小鼠感染后肺指数;明显减轻肺组织炎性损伤,降低肺组织病毒载量。在各时间点,与模型组相比明显低表达(P<0.05),以银屏散组效果最佳。结论:扶正祛邪双表法在治疗小鼠病毒性肺炎具有一定的优势。
Objective: To explore the therapeutic effect of Fuzhengqixue double table method on mice with viral pneumonia caused by influenza virus infection. Methods: Kunming mice were intranasally infected with influenza A / FM / 47 (H1N1) murine lung-adapted strain and treated with Jiexiao Powder (Yinqiao Powder), Gutai Powder (Yupingfeng San) Scattered + Yinqiaosan), silver screen San, positive control drug (ribavirin) intervention in the corresponding group of mice. The lung index and the inhibition rate were calculated at 3, 5, and 7 days after infection with influenza virus. The pathological changes were observed under the light microscope. The real-time RT-PCR was used to detect the viral load The amount of change. Results: Yinqiao powder, Yupingfeng powder and Yinpansan powder could significantly reduce the lung index after infection on the 3rd, 5th, and 7th day after infection. It could obviously reduce the inflammatory injury of lung tissue and reduce the viral load of lung tissue. At each time point, it was significantly lower than that of the model group (P <0.05), and Yinping Powder group had the best effect. Conclusion: Fuzhengqixue double table in the treatment of viral pneumonia in mice has some advantages.