Objective: To observe the changes of ischemic myocardial ceils apoptosis in rats following intervention with Xuefu Zhuyu Oral Liquid(血府逐瘀口服液,XFZY),as well as changes of protein expression of silent information regulator 1 (SIRT1) and SIRT1 pathway-related genes.Methods: H9c2 rat myocardial cells were divided into 6 groups: control group,oxygen glucose deprivation (OGD) group,SIRT1 siRNA group,OGD+SIRT1 siRNA group,OGD+XFZY group,and OGD+SIRT1 siRNA+XFZY group.Quantitative fluorescent polymerase chain reaction (PCR) and Westem blot were used to detect the concentration variations of SIRT1 and its pathway-related genes and corresponding protein expression after XFZY intervention and SIRT1 transfection.Results: Compared with the control group,the mRNA and protein expressions of SIRT1 were decreased obviously,while the mRNA and protein levels of P53,forkhead box protein O1 (FoxO1),FoxO3,FoxO4 and nuclear factor kappa B (NF-κB) were increased in the OGD group,SIRT1 siRNA group,and OGD+SIRT1 siRNA group (P＜0.01).Compared with the OGD group and OGD+SIRT1 siRNA group,the treatment of XFZY inhibited the decline in SIRT1 mRNA and protein expressions (P＜0.01),and down-regulated the mRNA and protein levels of P53,FoxO1,FoxO3,FoxO4 and NF-K B,respectively (P＜0.05 or P＜0.01).Conclusion: XFZY could prevent myocardial cells apoptosis probably by increasing the mRNA and protein expressions of SIRT1 and inhibiting the mRNA and protein expressions of P53,NF-κ B,FoxO1,FoxO3 and FoxO4.