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本研究以人胎胰进行组织培养和冻存,结果表明体外培养4～7天后的培养液中胰岛素含量高且恒定,胰腺组织在-196℃的条件下冻存180～250天,复苏后仍然具有较强的分泌胰岛素的活性,可供临床移植之用。本实验解决了临床应用的供需矛盾,为深入开展这方面的实验和临床研究,建立活组织细胞供应库打下了良好的基础。
In this study, human fetal pancreatic tissue culture and cryopreservation, the results show that cultured in vitro 4 to 7 days after the high insulin content of the medium and constant, the pancreas was stored at -196 ℃ for 180 to 250 days, after the recovery is still Has a strong secretion of insulin activity, for clinical transplantation purposes. This experiment solves the contradiction between supply and demand in clinical application, which lays a good foundation for further carrying out experimental and clinical research in this field and establishing a library of living tissue cells.