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目的 了解风湿性心脏病患者血清及γ-干扰素对瓣膜成纤维细胞胶原合成的影响 ,探讨转化生长因子β(TGF- β)在瓣膜纤维化中可能起的作用和 γ-干扰素用于预防及治疗瓣膜纤维化的可能性。 方法 取 5例风湿性心脏病患者的二尖瓣和主动脉瓣成纤维细胞进行体外培养并传代 ,将培养的每一例患者的二尖瓣、主动脉瓣细胞分为 7组 ,每组均为 5例。用 [2 ,3- 3H]脯氨酸掺入法测定各组细胞的胶原合成。 结果 患者自身血清使培养的瓣膜成纤维细胞胶原合成明显增加 ,这一作用能被 TGF- β中和抗体抑制 ,γ-干扰素能显著抑制瓣膜成纤维细胞的胶原合成。结论 风湿性心脏病患者血清中活性 TGF-β升高可能在瓣膜纤维化中起重要作用 ,γ-干扰素有可能用于临床预防或减轻瓣膜纤维化。
Objective To investigate the effects of serum and γ-interferon on collagen synthesis in rheumatic heart disease (CHF) patients and to explore the possible role of transforming growth factor-β (TGF-β) in valvular fibrosis and γ-interferon And the possibility of treating valve fibrosis. Methods Five patients with rheumatic heart disease were treated with mitral and aortic valve fibroblasts in vitro and passaged. The mitral and aortic valve cells of each patient were divided into 7 groups, each group was 5 cases. The [2, 3 - 3H] proline incorporation method was used to determine the collagen synthesis in each group of cells. Results Serum of patients significantly increased collagen synthesis of cultured fibroblasts, which was inhibited by TGF-β neutralizing antibody. Γ-interferon could significantly inhibit the collagen synthesis of valve fibroblasts. Conclusions Elevated serum levels of active TGF-β in patients with rheumatic heart disease may play an important role in valvular fibrosis. Γ-interferon may be used for clinical prevention or reduction of valve fibrosis.