自1960年Cocking发明酶法游离高等植物原生质体以来,植物原生质体的研究得到了迅速发展。现已有61种植物能从原生质体再生成植株,通过融合还得到了一些种内、种间乃至属间的体细胞杂种植株。原生质体技术的发展为植物引入外源DNA、染色体以及细胞器提供了一个良好的实验体系,无疑它将为实现植物的遗传改造(遗传工程) 奠定了基础。 Since 1960, Cocking invented enzymatic free higher plant protoplasts, plant protoplasts has been rapid development. There are 61 species of plants that can be regenerated from protoplasts. Some hybrids of somatic, interspecific and even intergeneric somatic hybrids have also been obtained. The development of protoplast technology provides a good experimental system for plants to introduce foreign DNA, chromosomes and organelles, which undoubtedly will lay the foundation for the genetic engineering (genetic engineering) of plants.