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目的:研究3种姜黄色素对体外培养人肝癌细胞HepG2 PPARγ蛋白的作用。方法:MTT法检测3种姜黄色素对体外培养人肝癌细胞HepG2的抑制作用;酶联免疫吸附检测人肝癌细胞HepG2 PPARγ蛋白的量;免疫组化法检测三种姜黄色素对人肝癌细胞的作用。结果:3种姜黄色素在高浓度时对人肝癌细胞HepG2有明显的抑制作用(P<0.01);且在4~16μg·mL-1的剂量范围内,随浓度升高抑制效果呈剂量依赖关系。在高浓度时,二脱甲氧基姜黄素比一脱甲氧基姜黄素组抑制作用好(P<0.001);3种姜黄色素都能增加PPARγ蛋白的量,结论:三种姜黄素均能激活PPARγ蛋白活性,这可能也是它体外抑制肝癌细胞HepG2生长的机理之一。
Objective: To study the effects of three curcuminoids on HepG2 PPARγ protein in vitro. Methods: MTT assay was used to detect the inhibitory effect of three curcumin on HepG2 cells in vitro. The amount of HepG2 PPARγ protein was detected by enzyme-linked immunosorbent assay (ELISA) and the effect of three curcumin on human hepatoma cells was detected by immunohistochemistry. Results: The three curcuminoids significantly inhibited HepG2 cells in high concentration (P <0.01), and the dose-dependent inhibition of the three curcumin concentrations in the range of 4 ~ 16 μg · mL-1 dose-dependently . At high concentrations, didemethoxy curcumin had a better inhibitory effect than the demethoxycurcumin group (P <0.001). All three curcuminoids increased the amount of PPARγ protein. Conclusions: All three curcumin Activation of PPARγ protein activity, which may also be one of the mechanisms of its inhibition of HepG2 liver cancer cell growth in vitro.