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目的:探讨乌司他丁(UTI)对细菌性重症脓毒症大鼠血清白细胞介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)水平的调控作用。方法:将SD大鼠经腹腔内注射创伤弧菌建立脓毒症模型,并随机分为3组:生理盐水对照组、氧氟沙星抗感染组、乌司他丁+氧氟沙星抗炎抗感染组,每组15只。15 h后取单侧颈总动、静脉血标本测定血清IL-10、TNF-α、IL-1β的水平。结果:合用乌司他丁的抗炎抗感染组较单纯氧氟沙星抗感染组及生理盐水对照组IL-10水平显著增高(P<0.01),而TNF-α、IL-1β水平则显著降低(P<0.01);IL-10与TNF-α、IL-1β水平呈线性负相关。结论:乌司他丁能上调脓毒症大鼠抗炎因子IL-10水平,并可下调炎症因子TNF-α、IL-1β,阻断炎症反应,改善预后。
Objective: To investigate the effects of ulinastatin on serum interleukin-10 (IL-10), tumor necrosis factor-α (TNF-α) and interleukin-1β (IL- 1β) level of regulation. Methods: SD rats were injected intraperitoneally with Vibrio vulnificus to establish sepsis model and randomly divided into 3 groups: saline control group, ofloxacin anti-infection group, ulinastatin + ofloxacin anti-inflammatory Anti-infection group, each group of 15. Fifteen hours later, the levels of IL-10, TNF-α and IL-1β in serum were measured by unilateral common carotid and venous blood samples. Results: The levels of IL-10 in the anti-inflammatory and anti-infective groups combined with ofloxacin and the saline control group were significantly increased (P <0.01), while the levels of TNF-α and IL-1β were significantly higher (P <0.01). There was a linear negative correlation between IL-10 and TNF-α and IL-1β levels. Conclusion: Ulinastatin can upregulate the level of anti-inflammatory cytokine IL-10 in septic rats, and can down-regulate inflammatory cytokines TNF-α and IL-1β to block the inflammatory response and improve the prognosis.