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以蛋白激酶Ａ的一磷酸化底物肽ＬＲＲＡＳＬＧ 为模型肽， 研究了硫代磷酸化及荧光标记反应条件。荧光标记试剂５｛［（（２碘代乙酰） 氨）乙基］氨基｝萘１磺酸（１，５ＩＡＥＤＡＮＳ） 的适宜浓度为１．６ ｍ ｍｏｌ／Ｌ， 标记反应缓冲液的适宜ｐＨ 为７ 至８ 。实验了标记肽分别在Ｎ端序列分析、电喷雾质谱和０ ．１％ 三氟乙酸存在下的稳定性。比较了标记肽和未标记肽的紫外吸收光谱的差异特征。初步显示高效液相色谱蛋白酶解肽谱分析中， 荧光检测硫代磷酸化荧光标记肽的可能性。
Using the phosphorylated substrate peptide LRRASLG of protein kinase A as a model peptide, the thiophosphorylation and fluorescent labeling reaction conditions were studied. Fluorescent labeling reagent 5 {((2 iodoacetyl) amino) ethyl] amino} naphthalene 1 sulfonic acid (1,5 IAEDANS) suitable concentration of 1.6 m mol / L, labeled reaction buffer The optimum pH of the solution is 7 to 8. Labeled peptides were N-terminal sequence analysis, electrospray mass spectrometry and 0. 1% trifluoroacetic acid in the presence of stability. The differences in the UV absorption spectra of labeled peptides and unlabelled peptides were compared. It is preliminary shown that the possibility of fluorescence detection of thiophosphorylated fluorescent labeled peptide in high performance liquid chromatography (GPC) peptide proteome analysis.