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目的:探讨番茄红素对豚鼠离体心脏缺血/再灌注损伤的影响。方法:将30只豚鼠离体心脏随机分为5组:正常组、缺血/再灌注组(Model)、番茄红素低剂量组(2.5 mg·L-1)、番茄红素中剂量组(5 mg·L-1)、番茄红素高剂量组(10 mg·L-1),模型组全心缺血40 min之后,K-H液继续灌注60 min,治疗组的灌流液中加入不同质量浓度的番茄红素。观察的指标有:心率、冠脉流量、梗死面积、灌流液中乳酸脱氢酶(LDH)含量、心肌组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)的活性。结果:各组在再灌注后各项指标均发生变化,在再灌注60 min时,模型组与正常组比较心率下降(125±9),(169±16)次/min(P<0.01);灌流量下降(3.48±0.43),(5.53±0.30)mL·min-1(P<0.01);梗死面积显著增加(19.57±2.34),(1.57±0.29)%(P<0.01);LDH漏出量增加(206.67±11.85),(120.96±13.56)U·L-1(P<0.01);SOD活性下降(99.14±8.24),(153.22±8.93)U·mg-1(P<0.01),MDA含量增加(6.17±0.73),(3.80±0.41)μmol·g-1(P<0.01)。与模型组比较,番茄红素各组心率和冠脉流量增加,梗死面积减少,LDH漏出量减少,心肌组织中MDA含量降低,SOD活性增加,各剂量组之间呈剂量依赖性。结论:番茄红素对豚鼠离体心脏缺血/再灌注损伤有保护作用,其作用机制可能与抗氧化应激相关。
Objective: To investigate the effect of lycopene on isolated guinea pig ischemia / reperfusion injury. Methods: Thirty guinea pigs were randomly divided into 5 groups: normal group, model of ischemia / reperfusion, low dose of lycopene (2.5 mg · L-1) and medium dose of lycopene 5 mg · L-1) and high-dose lycopene group (10 mg · L-1). After the model group was given complete ischemia for 40 min, KH solution was continuously perfused for 60 min. The perfusion solution of the treatment group was given different concentrations of Lycopene. Observed indicators include: heart rate, coronary flow, infarct size, lactate dehydrogenase (LDH) content in perfusion fluid, malondialdehyde (MDA) content and superoxide dismutase (SOD) activity in myocardial tissue. Results: The indexes of all groups changed after reperfusion. At 60 minutes after reperfusion, the heart rate of model group decreased (125 ± 9) and (169 ± 16) times / min (P <0.01) compared with that of normal group. The perfusion decreased (3.48 ± 0.43), (5.53 ± 0.30) mL · min-1 (P <0.01), the infarct size increased significantly (19.57 ± 2.34) and (1.57 ± 0.29)% (P <0.01). The SOD activity decreased (99.14 ± 8.24), (153.22 ± 8.93) U · mg-1 (P <0.01) (6.17 ± 0.73) and (3.80 ± 0.41) μmol · g-1, respectively (P <0.01). Compared with the model group, the increase of heart rate and coronary flow in each group of lycopene decreased infarct size, decreased LDH leakage, reduced myocardial MDA content, increased SOD activity, and dose-dependent manner. CONCLUSION: Lycopene has a protective effect on guinea pig ischemia-reperfusion injury in vitro and its mechanism may be related to anti-oxidative stress.