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[目的 ]了解妊娠中晚期和哺乳期母体接触磺胺二甲嘧啶对子代大鼠空间辨别能力及脑内胆碱乙酰转移酶(ChAT)活性的影响。 [方法 ]SD大鼠在受孕第 7天起至哺乳期结束分别每日给予磺胺二甲嘧啶 0、5 0、10 0和 2 0 0mg/kg ,用水迷宫实验检测各组 2 0～ 3 0日龄仔鼠的空间辨别能力 ,同时取 3 0日龄仔鼠的大脑进行免疫组化染色 ,观察脑内ChAT的表达。 [结果 ]染毒组仔鼠出现空间辨别能力障碍 ,尤其是 10 0、2 0 0mg/kg剂量组与对照组相比到达终点时间显著延长 ,错误次数显著增多 (P <0 .0 5 )。大脑的免疫组化分析发现 ,染毒组仔鼠大脑皮质和海马区ChAT的表达降低 ,尤其是10 0、2 0 0mg/kg剂量组与对照组相比差异有统计学意义 (P <0 .0 5 )。 [结论 ]给围生期母鼠喂饲磺胺二甲嘧啶可能通过降低大鼠体内的甲状腺素浓度 ,影响脑内ChAT的水平 ,最终造成仔鼠对空间辨别能力的学习和记忆障碍
[Objective] To investigate the effect of maternal exposure to sulfamethazine on spatial differentiation and choline acetyltransferase (ChAT) activity in the offspring in the second trimester of pregnancy. [Methods] From the 7th day of conception to the end of lactation, SD rats were given sulfamethazine 0.5, 10, 10 and 200 mg / kg daily, and the water maze test was used to detect each group from 20 to 30 days At the same time, 30-day-old pups’ brains were subjected to immunohistochemical staining to observe the expression of ChAT in the brain. [Result] Disability discrimination was found in the offspring rats. Especially, the time to reach the end point was significantly prolonged and the number of mistakes significantly increased (P <0.05) compared with the control group at dose of 10 0, 200 mg / kg. Immunohistochemical analysis of the brain showed that the expression of ChAT in the cerebral cortex and hippocampus in the exposed group was decreased, especially in the dose of 10,200 mg / kg compared with the control group (P <0. 0 5). [Conclusion] The feeding of sulfamethazine to the perinatal female rats may affect the level of ChAT in the brain by decreasing the concentration of thyroxine in the rat, eventually resulting in the learning and memory impairment of the pups’ ability to distinguish the space