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目的 :探讨玄参提取物 (SnPs)对局灶性缺血后脑组织的保护作用及对脑血流量的影响。方法 :雄性SD大鼠 (2 4 2±s 7)g 4 0只随机分 5组 (均n =8) :假手术组、模型对照组、3个不同剂量SnPs治疗组 (1,5 ,10mg·kg- 1尾静脉注射 )。大脑中动脉线栓法建立局灶性脑缺血模型 (MCAO) ,缺血 2 4h后 ,用神经功能评分和梗死体积改变来评价SnPs对脑缺血的治疗作用。另将MCAO模型缺血SD大鼠分成 4组 (模型对照组n =8,3个不同剂量 1,5 ,10mg·kg- 1SnPs治疗组均n =6 )用于测脑血流量 ,用激光多普勒仪分别在 6个时间点 (缺血前 ,缺血即刻 ,缺血后 30min和 2h ,以及缺血 2h再灌 30min ,2h)记录双侧的皮层血流量。结果 :脑缺血 2 4h后 ,各SnPs治疗组与模型对照组相比 ,脑梗死体积明显减少 (P <0 .0 5 ) ,神经功能均明显改善 (P <0 .0 1) ;但在此剂量范围内无明显的剂量依赖性。各SnPs治疗组与模型组相比 ,缺血 2h后皮层CBF明显改善 (P <0 .0 5 ) ,其中 5mg·kg- 1剂量组对于缺血各时间点的血流改善均有显著作用 (P <0 .0 5 )。结论 :SnPs对于脑缺血损伤具有保护作用 ,此作用可能与提高脑血流量有关。
Objective: To investigate the protective effect of Scrophulariaceae extract (SnPs) on focal cerebral ischemia and its effect on cerebral blood flow. METHODS: Male Sprague-Dawley rats (2 4 2±s 7) g 40 were randomly divided into 5 groups (all n = 8): sham operation group, model control group, and 3 different doses of SnPs treatment group (1, 5, 10 mg) · kg-1 tail vein injection). Middle cerebral artery occlusion was performed to establish a focal cerebral ischemia model (MCAO). After 24 hours of ischemia, neurological function scores and infarct volume changes were used to evaluate the therapeutic effects of SnPs on cerebral ischemia. In addition, MCAO model ischemic SD rats were divided into 4 groups (model control group n = 8, 3 different doses 1, 5, 10mg · kg-1SnPs treatment group were n = 6) for measuring cerebral blood flow, with more laser Puller blood flow was recorded bilaterally at 6 time points (pre-ischemia, immediate ischemia, 30 min and 2 h after ischemia, and 2 h after ischemia, and 30 min after 2 h). RESULTS: After 24 hours of cerebral ischemia, the volume of cerebral infarction was significantly reduced in each SnPs treatment group compared with the model control group (P < 0.05), and neurological function was significantly improved (P <0. 01); but There is no significant dose dependence within this dose range. Compared with the model group, the cortical CBF of the SnPs treatment group was significantly improved after 2h ischemia (P < 0.05), and the 5mg · kg-1 dose group had a significant effect on the improvement of blood flow at each time point of ischemia ( P < 0 .0 5 ). Conclusion: SnPs have a protective effect on cerebral ischemic injury. This effect may be related to the increase of cerebral blood flow.