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目的:探讨阿片类生长因子受体假基因1（OGFRP1）对卵巢癌细胞增殖、侵袭和迁移的影响及其作用机制。方法:选取120例卵巢癌患者癌组织及正常卵巢组织，实时荧光定量PCR（RT-qPCR）检测OGFRP1和miR-665表达水平；将A2780细胞分为空白（PBS）组、si-NC组、si-OGFRP1组、miR-NC组、miR-665组、si-OGFRP1+anti-miR-NC组、si-OGFRP1+anti-miR-665组。四甲基偶氮唑盐比色法（MTT）检测细胞存活率；Transwell检测细胞迁移和侵袭；双荧光素酶报告实验检测OGFRP1和miR-665的靶向关系。结果:与正常卵巢组织相比，卵巢癌组织中OGFRP1表达水平升高（2.75±0.27 vs 1.00±0.09），miR-665表达水平降低（0.51±0.05 vs 1.00±0.08）（均n P<0.05）。抑制OGFRP1表达或过表达miR-665后，卵巢癌A2780细胞的活性、细胞迁移与侵袭数均降低（均n P<0.05）。双荧光素酶报告实验结果显示，OGFRP1靶向调控miR-665；抑制miR-665和OGFRP1表达后，卵巢癌A2780细胞活性升高，迁移、侵袭细胞数增加（n P<0.05）。n 结论:抑制OGFRP1表达可抑制卵巢癌A2780细胞增殖、迁移和侵袭，其机制可能与上调miR-665有关。“,”Objective:To investigate the effect of OGFRP1 on proliferation, invasion and migration of ovarian cancer cells and the underlying mechanism.Methods:120 pairs of cancer tissues and normal ovarian tissues from patients with ovarian cancer were included. Real-time fluorescent quantitative PCR (RT-qPCR) was used to detect the expression levels of OGFRP1 and miR-665. A2780 cells were divided into the blank (PBS) group, si-NC group, si-OGFRP1 group, miR-NC group, miR-665 group, si-OGFRP1+anti-miR-NC group, and si-OGFRP1+anti-miR-665 group. Methyl-thiazolyl tetrazolium (MTT) - based colorimetric method was used to detect cell viability; Transwell assay was used to detect cell migration and invasion; dual luciferase reporter assay was used to detect the targeting relationship between OGFRP1 and miR-665.Results:Compared with normal ovarian tissue, the expression level of OGFRP1 was increased (2.75±0.27 vs 1.00±0.09) , and the expression level of miR-665 was decreased (0.51±0.05 vs 1.00±0.08) (all n P<0.05) , in ovarian cancer tissue. After inhibiting the OGFRP1 expression or overexpressing miR-665, the viability, cell migration and invasion of ovarian cancer A2780 cells were reduced (alln P<0.05) . Dual luciferase report assay showed that OGFRP1 targeted and regulated miR-665. After inhibiting the expression of miR-665 and OGFRP1, the viability of ovarian cancer A2780 cells increased, and so did the number of migrating and invasive cells (n P<0.05) .n Conclusion:Suppressing the expression of OGFRP1 can inhibit the proliferation, migration and invasion of ovarian cancer A2780 cells. The underlying mechanism may be related to the up-regulation of miR-665.