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目的 :研究抗抑郁剂相关的基因。方法 :采用不同应激方式交替持续应激 2 0d造成大鼠慢性应激损伤模型 ,提取大鼠海马组织总RNA ,行逆转录聚合酶链反应。PCR产物上样于聚丙烯酰胺凝胶 ,银染法分离差异表达的条带 ,用斑点杂交方法对差异表达片段进行验证 ,将表达量有差异的条带测序。结果 :与慢性应激组相比 ,地昔帕明可以上调微管相关蛋白基因的表达 ,氟西汀可以上调一种神经元肌动蛋白结合蛋白树状棘蛋白的基因表达。结论 :结果提示地昔帕明与氟西汀可能通过上调中枢神经系统与神经再生有关的蛋白的基因表达而发挥抗抑郁作用
Objective: To study antidepressant-related genes. Methods: The model of chronic stress injury was induced by alternating different stress modes for 20 days in rats. Total RNA was extracted from the hippocampus of rats and subjected to reverse transcription polymerase chain reaction. The PCR products were loaded on polyacrylamide gel, and the differentially expressed bands were isolated by silver staining. The differential expression fragments were verified by dot blot hybridization, and the bands with different expression levels were sequenced. RESULTS: Desipramine increased microtubule-associated protein gene expression compared with chronic stress, and fluoxetine up-regulated the expression of a neuronal actin-binding protein dendritic protein. Conclusions: The results suggest that desipramine and fluoxetine may exert anti-depressant effects by up-regulating the gene expression of the proteins involved in central nervous system and nerve regeneration