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目的探讨芹菜素对人卵巢癌CAOV3细胞增殖的抑制作用。方法2006年10月至12月在中国医科大学附属第一医院肿瘤研究所应用四甲基偶氮唑蓝(MTT)比色法检测芹菜素对CAOV3细胞增殖的抑制作用,流式细胞仪技术分析芹菜素对CAOV3细胞增殖周期的影响。结果20～160μmol/L芹菜素均能抑制CAOV3细胞的生长,且呈明显的时间、剂量依赖性关系。细胞周期分析显示芹菜素组G2/M期细胞比例明显增多,呈量效关系。结论芹菜素抑制CAOV3细胞增殖,可能通过使CAOV3细胞停滞在G2/M期而诱导其凋亡。
Objective To investigate the inhibitory effect of apigenin on the proliferation of human ovarian cancer cell line CAOV3. Methods The inhibitory effect of apigenin on the proliferation of CAOV3 cells was detected by MTT assay from October 2006 to December in Cancer Institute of the First Affiliated Hospital of China Medical University. Flow cytometry Effect of Apigenin on the Proliferation Cycle of CAOV3 Cells. Results Apigenin 20 ~ 160μmol / L could inhibit the growth of CAOV3 cells in a time-and dose-dependent manner. Cell cycle analysis showed apigenin group G2 / M phase cells increased significantly, showing a dose-response relationship. Conclusions Apigenin can inhibit the proliferation of CAOV3 cells and induce its apoptosis by arresting the cells in G2 / M phase.