目的　了解重组人肝再生增强因子 (hALR)对实验性肝纤维化基质分解素 1(MMP3 )基因表达的影响。方法　建立四氯化碳 (CCl4 )中毒性及人血白蛋白免疫损伤性两种大鼠肝纤维化模型 ,在造模的同时给予不同剂量的重组hALR。在不同的时间点留取大鼠肝组织标本 ,提取总RNA ,用RT PCR测定MMP3 的基因表达水平。结果　在两种模型中 ,hALR治疗组大鼠肝组织MMP3 的基因表达水平在模型形成的不同阶段均明显高于模型组 ;高剂量hALR组大鼠肝组织MMP3 的基因表达水平均明显高于低剂量组。结论　重组hALR可能有促进大鼠实验性肝纤维化MMP3 基因表达的作用。 Objective To investigate the effect of recombinant human augmenter of liver regeneration (hALR) on the expression of matrix metalloproteinase-3 (MMP-3) in experimental hepatic fibrosis. Methods Liver fibrosis induced by carbon tetrachloride (CCl4) and immunosuppressive human albumin were established in rats. Different doses of recombinant hALR were given at the same time. Rat liver tissue samples were taken at different time points and total RNA was extracted. The gene expression level of MMP3 was determined by RT PCR. Results In both models, the gene expression of MMP3 in liver tissue of rats in hALR treatment group was significantly higher than that in model group at different stages of model formation. The gene expression of MMP3 in liver tissue of rats in high-dose hALR group was significantly higher than that in model group Dose group. Conclusion Recombinant hALR may promote the expression of MMP3 gene in experimental hepatic fibrosis in rats.