近十年来,鲎试验已广泛用以检测注射用药物和医疗器具中的细菌内毒素,在某些国家还用来部分代替家兔的发热试验。目前常用的试管法虽结果判断容易,方法简单,但试剂用量过大,成本较高。其它一些改良法如微量玻片法、微量稀释板法、放射同位素法及基质成色法等虽所用试剂较少,但结果不易判断,方法也较复杂。作者在毛细管法的基础上提出了一种改良法。试剂:将冻干的鲎变形细胞溶解物重新融化,保存在-20℃冰箱中(时间不得超过4周)。将标准大肠杆菌、克雷伯氏菌内毒素,流感疫苗(作参考内毒素)和未知内毒素含量的假单胞菌苗(预先稀释1×10~(-5)分别用蒸馏水稀释到1.0 ng/ml,-70℃贮存,待用。以0.1%溴麝香草酚蓝酒精/磷酸盐缓冲液 In the last decade, test 鲎 has been widely used to detect bacterial endotoxin in injectable drugs and medical devices, and in some countries it is also used in part to replace the fever test in rabbits. Although the commonly used test tube method is easy to determine the result, the method is simple, but the reagent dosage is too large and the cost is high. Some other modified methods such as micro-slide method, micro-dilution plate method, radioisotope method and matrix method, although less used reagents, but the results are not easy to judge, the method is more complicated. The author proposed a modified method based on the capillary method. Reagent: The lyophilized goblet cell lysate is thawed and stored in a freezer at -20 ° C for up to 4 weeks. Pseudomonas aeruginosa (standard dilution of 1 × 10 ~ (-5)) of standard E. coli, Klebsiella LPS, influenza vaccine (reference endotoxin) and unknown endotoxin were diluted to 1.0 ng with distilled water / ml, stored at -70 ℃, stand with 0.1% bromothymol blue alcohol / phosphate buffer