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玉米黄质环氧化酶(zeaxanthin epoxidase,ZEP)在植物脱落酸(abscisic acid,ABA)生物合成间接途径中发挥着重要调节作用。该研究根据太子参转录组数据,克隆获得太子参的Pseudostellaria heterophylla玉米黄质环氧化酶基因,命名Ph ZEP。对其序列进行生物信息学分析,结果表明Ph ZEP的开放阅读框(open reading frame,ORF)为1 263 bp,编码420个氨基酸,预测其蛋白的相对分子质量为47.34 k Da,等电点(theoretical p I)为6.64;具有脂质蛋白附着点和黄素蛋白单加氧酶的特征性结构域,且在N端含有分泌信号肽。实时荧光PCR分析发现,Ph ZEP在叶片中表达量最高,其次是茎和须根;Ph ZEP在盛花期后10,40 d块根中的表达量较高;ABA处理后Ph ZEP表达量与对照组相当,经氟啶酮处理后Ph ZEP的表达量显著高于对照组。该研究从太子参中分离鉴定了ZEP基因,对后续研究其基因的功能特点和解析ABA在太子参生长发育过程的遗传调控提供研究基础。
Zeaxanthin epoxidase (ZEP) plays an important regulatory role in the indirect pathway of plant biosynthesis of abscisic acid (ABA). In this study, the Pseudostellaria heterophylla zeaxanthin cyclooxygenase gene from Pseudostellaria heterophylla was cloned and cloned according to the transcript data of P. heterophylla, and named Ph ZEP. The results of bioinformatics analysis showed that the open reading frame (ORF) of Ph ZEP was 1 263 bp, encoding 420 amino acids. The predicted relative molecular mass of the protein was 47.34 k Da. The isoelectric point theoretical p I) was 6.64; a characteristic domain with a lipid protein attachment site and flavoprotein monooxygenase and a secretion signal peptide at the N-terminus. Real-time PCR analysis showed that Ph ZEP was highest in leaves, followed by stems and fibrous roots. Ph ZEP was higher in tubers at 10 and 40 days after flowering; Ph ZEP expression was comparable with that in control , The expression of Ph ZEP after fluoridone treatment was significantly higher than that of the control group. In this study, the ZEP gene was isolated and identified from Pseudostellaria heterophylla, which provided the basis for further studies on the functional characteristics of the gene and the genetic control of ABA during the growth and development of Pseudostellaria heterophylla.