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用错配聚合酶链反应（Ｍ－ＰＣＲ）及限制性片段长度多态性分析（ＲＦＬＰ）方法，对９７例慢性乙肝病毒感染（ＨＢＶ）者血中ＨＢＶＤＮＡ前Ｃ区Ａ８３基因突变进行检测。结果显示：共检出Ａ８３基因突变株感染３７例（３８．１％），其中重症肝炎和重度慢性肝炎患者的检出率均明显高于轻度慢性肝炎和ＨＢＶ携带者；ＡＬＴ明显升高者的检出率明显高于ＡＬＴ正常或升高不明显者；ＨＢｅＡｂ（＋）者明显高于ＨＢｅＡｇ（＋）者。表明Ａ８３基因突变株感染是导致部分病人病情加重和病变活动的原因之一
The mutation of A83 gene in pre-HBVDNA of 97 patients with chronic hepatitis B virus infection was detected by mismatch polymerase chain reaction (M-PCR) and restriction fragment length polymorphism (RFLP). The results showed that 37 cases (38.1%) were infected with A83 gene mutation, and the detection rates of severe hepatitis and severe chronic hepatitis were significantly higher than those of mild chronic hepatitis and HBV carriers. The ALT was significantly higher The detection rate was significantly higher than that of ALT normal or not obvious; HBeAb (+) was significantly higher than that of HBeAg (+). The results showed that the infection of A83 mutant was one of the causes of aggravating and pathological changes in some patients