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目的 研究番茄红素及维生素E对燃煤型氟中毒致大鼠睾丸组织中Clusterin蛋白表达的影响及机制.方法 60只雄性SD大鼠分为低、中、高氟组、高氟+维生素E组、高氟+番茄红素组和对照组,每组10只.染毒组喂饲含不同比例的燃煤型氟中毒病区煤烘玉米的饲料,构建燃煤型氟中毒动物模型,高氟+维生素E组和高氟+番茄红素组分别喂饲添加维生素E和番茄红素的病区煤烘玉米配制饲料,对照组给予正常饲料.观察各组大鼠氟斑牙情况;氟离子选择电极法检测大鼠尿和睾丸组织氟含量;HE染色光镜下观察睾丸组织病理学改变;原位缺口末端标记(TUNEL)染色观察生精细胞凋亡情况;免疫组化法检测大鼠睾丸组织中Clusterin蛋白表达.结果 与对照组相比,低、中、高氟组大鼠氟斑牙严重程度、尿氟及睾丸组织氟含量、睾丸组织病理学损伤程度和睾丸组织生精细胞凋亡指数均依次增加(P＜0.05),各氟剂量组上述指标随氟剂量的增大而增大;与高氟组相比,高氟+维生素E组和高氟+番茄红素组上述指标明显降低;与对照组相比,低、中、高氟组大鼠Clusterin蛋白水平依次下降(P＜0.05),各氟剂量组Clusterin蛋白表达水平随氟剂量的增大而下降.与高氟组相比,高氟+维生素E组和高氟+番茄红素组Clusterin蛋白水平明显升高.结论 燃煤型氟中毒可引起大鼠睾丸组织的性生殖损害,生精细胞凋亡率增加,Clusterin蛋白的表达降低.抗氧化剂可显著降低生殖细胞凋亡率,提高Clusterin蛋白的表达,改善雄性生殖功能的损伤.“,”Objective To study the effect of lycopene and vitamin E on the expression of clusterin protein in fluorosis rats caused by coal-burning.Methods The SD rats were divided into high-,.medium-and low-doses of fluorine groups,high dose of fluorine +lycopene group,high dose of fluorine + vitamin E group and normal control group.The rats of control group were treated with normal fodders.The high-,medium-and low-doses of fluorine groups were treated with fodders that with different proportions of fluorine respectively.The rats of high dose of fluorine + lycopene groupwere treated with fodders that with high proportions of fluorine and lycopene.The rats of high dose of fluorine +vitamin E groupwere treated with fodders that with high proportions of fluorine and vitamin E.After 120d,dental fluorosis was determined by observation,urinary fluoride and testes fluoride were detected by fluorine ion-selective electrode.Hematoxylin and eosin (HE) stain was used to examine the degree of testes injury.The apoptotic index of spermatogenic cell was measured by TUNEL.The expression of clusterin was measuredby immuohistochemistry.Results Compared with the control group,the dental fluorosis,urinary fluoride,testes fluoride,the degree of testes injury and the apoptotic index of spermatogenic cell in high-,medium-and low-doses of fluorine groups were significantly higher (P＜0.05),and these indicators significantly increased in a dose-dependent manner (P＜0.05).Compared with the high dose of fluorine group,the dental fluorosis,urinary fluoride,testes fluoride,the degree of testes injury and the apoptotic index of spermatogenic cell in high dose of fluorine +lycopene group and high dose of fluorine + vitamin E group were significantly lower (P＜0.05).Compared with the control group,the expressions of clusterin in high-,medium-and low-doses of fluorine groups were significantly lower (P＜0.05),and The expressions of clusterin significantly decreased in a dose-dependent manner (P＜0.05).Compared with the high dose of fluorine group,the expressions of clusterin in high dose of fluorine +lycopene group and high dose of fluorine + vitamin E group were significantly higher (P＜0.05).Conclusion Coal-burning caused fluorosis may leads to increased apoptotic index of rats's permatogenic cell,decreased clusterin expression.The antioxidants can decrease apoptotic index of rats' s permatogenic cell,increase clusterin expression,and decrease the degree of testes injury.