以肼基二硫代甲酸甲酯 ( )与相应的酮或醛反应 ,生成 3-二取代甲撑基二硫代甲酸甲酯 ( ) .化合物 与 N-单取代哌嗪反应 ,制得不同取代基的标题化合物缩胺硫脲 ( ) ,产率 2 7%～ 85 % .产物的结构用 IR,NMR和元素分析证实 ,用磺酰罗丹明 β蛋白染色法 ( SRB法 )和四氮唑盐还原法 ( MTT法 )检验了它们对鼠白血病 ( P-388)和人肺癌 ( A-5 4 9)细胞株的抑制活性 The reaction of methyl hydrazinodithiocarbamate with the corresponding ketone or aldehyde gives the methyl 3-disubstituted methyl dithiocarbamate (). The compound is reacted with N-monosubstituted piperazine to give various substitutions The title compound thiadiazine () was obtained in a yield of 27-85%. The structure of the product was confirmed by IR, NMR and elemental analysis with the sulforhodamine beta protein staining (SRB method) and the tetrazolium salt Their inhibitory activity on murine leukemia (P-388) and human lung cancer (A-5 49) cell lines was examined by the reduction method (MTT method)