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为筛选青翘抗炎的活性部位,明确作用机制,该研究给予雄性SD大鼠青翘水煎液、乙酸乙酯、正丁醇和剩余水层各部位提取物,连续15 d,复制急性肺损伤炎症模型,观察肺组织切片结构,测试肺泡灌洗液的IL-6,TNF-α,IL-1β,IL-10的水平,收集血清,采用1H-NMR代谢组学技术分析大鼠血清内源性代谢产物的变化规律。结果显示青翘乙酸乙酯部位抗急性肺损伤炎症活性较好,能够抑制炎症因子IL-6,TNF-α,IL-1β的释放,明显降低血清中肌酸、β-OH-丁酸、琥珀酸、赖氨酸、缬氨酸、异亮氨酸、谷氨酰胺的含量,升高GPC的含量。该研究从体内代谢的角度进一步证明了乙酸乙酯部位是青翘抗炎的主要活性部位,主要通过调节肌酸代谢、胆碱代谢、支链氨基酸代谢以及三羧酸循环等通路发挥抗炎作用,可为青翘抗炎药效物质基础的研究奠定基础。
In order to screen the anti-inflammatory active site of Atrazus curcumin, the mechanism of action was clarified. The extract of AQI, Ethyl Acetate, n-Butanol and the remaining water layer of male Sprague Dawley rats were harvested continuously for 15 days to replicate acute lung injury Inflammatory model was used to observe the structure of lung tissue. The levels of IL-6, TNF-α, IL-1β and IL-10 in BALF were measured. Serum was collected. Changes of sex metabolites. The results showed that the anti-Acute Lung Injury induced by Ethyl Acetate could inhibit the release of inflammatory cytokines IL-6, TNF-α and IL-1β and significantly reduce the levels of creatine, β-OH-butyric acid, Acid, lysine, valine, isoleucine, glutamine, increase the content of GPC. The study further proves that the ethyl acetate fraction is the main active site of the anti-inflammatory activity of P. americana from the perspective of metabolism in vivo and exerts anti-inflammatory effects mainly through regulating the pathways of creatine metabolism, choline metabolism, branched-chain amino acid metabolism and the tricarboxylic acid cycle , Which can lay the foundation for the study of the substance base of anti-inflammatory and anti-inflammatory drugs.