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目的探讨角蛋白8(Keratin 8,K8)在溃疡性结肠炎(Ulcerative Colitis,UC)的表达及其与疾病严重程度的关系。方法采用免疫组化Elivison plus方法检测30例UC内镜活检标本及10例正常对照石蜡组织中K8的表达情况,然后用IPP6.0(Imagepro-plus 6.0)专业图像分析软件定量分析其表达,并比较疾病严重程度不同的组织切片中表达量的差异。结果UC患者与正常对照组相比,平均光密度值(MOD值)高,即K8在UC患者肠道上皮表达增加,MOD分别为(0.0230.018)和(0.0080.005),P0.05;中重度UC患者与轻度相比,中重度患者表达低于轻度患者,MOD分别为(0.0170.015)和(0.0390.014),PO.05。结论K8可能在UC的发病中起一定作用。而且疾病严重程度不同,表达量亦有差异,且含量随病变加重而减低。该指标有可能成为判断UC疾病严重程度的指标。
Objective To investigate the expression of keratin 8 (K8) in ulcerative colitis (UC) and its relationship with disease severity. Methods Immunohistochemical Elivison plus method was used to detect the expression of K8 in 30 cases of UC endoscopic biopsy specimens and 10 cases of normal control paraffin tissues. The expression of K8 was analyzed by IPP6.0 (imagepro-plus 6.0) professional image analysis software. Differences in expression levels in different tissue sections of disease severity were compared. Results The mean optical density (MOD) value of UC patients was higher than that of the normal control group. The expression of K8 in the intestinal epithelium of UC patients was (0.0230.018) and (0.0080.005) respectively, P <0.05; Moderate and severe UC patients compared with mild, moderate and severe patients were lower than mild patients, MOD were (0.0170.015) and (0.0390.014), PO.05. Conclusion K8 may play a role in the pathogenesis of UC. And the severity of the disease is different, the expression levels are also different, and the content decreases with lesion. The index may become a judge of the severity of UC disease indicators.