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目的:探讨不同浓度淫羊藿苷、黄芩苷和大黄素对细菌内毒素脂多糖(lipopolysaccharide,LPS)刺激人牙龈成纤维细胞(human gingival fibroblasts,HGF)产生前列腺素E2(prostaglandin E2,PGE2)的影响。方法:以培养的人牙龈成纤维细胞为实验模型,用MTT试验检测淫羊藿苷、黄芩苷和大黄素抑制LPS对牙龈成纤维细胞毒性作用的效果,初步筛选其作用浓度;然后用酶联免疫法检测三种提取物对LPS刺激牙龈成纤维细胞产生重要的骨吸收因子PGE2的影响。结果:LPS对HGF有明显的细胞毒性,三种提取物均可显著提高细胞成活率。LPS作用6h后培养上清中PGE2含量上升,而同时加入淫羊藿苷、黄芩苷和大黄素各浓度组PGE2含量显著低于对照组(p<0.01)。结论:淫羊藿苷、黄芩苷和大黄素均可抑制LPS对HGF的细胞毒性作用,并且可抑制LPS刺激HGF产生PGE2。
Objective: To investigate the effect of different concentrations of icariin, baicalin and emodin on the production of prostaglandin E2 (PGE2) by human lipopolysaccharide (LPS)-stimulated human gingival fibroblasts (HGF). influences. METHODS: Using cultured human gingival fibroblasts as an experimental model, MTT assay was used to detect the effects of icariin, baicalin, and emodin on the toxicity of LPS to gingival fibroblasts. Immunoassay to detect the effect of three extracts on the production of bone resorption factor PGE2, an important bone resorption factor, in gingival fibroblasts stimulated by LPS. Results: LPS had obvious cytotoxicity to HGF, and all three extracts could significantly increase the cell survival rate. The content of PGE2 in the culture supernatant increased 6 h after LPS treatment, while the content of PGE2 in the icariin, baicalin, and emodin groups was significantly lower than that in the control group (p<0.01). CONCLUSION: Icariin, baicalin, and emodin can all inhibit the cytotoxic effect of LPS on HGF, and inhibit LPS from stimulating HGF to produce PGE2.