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目的通过基因表达谱研究寻找苯中毒发病机制中参与苯中毒免疫调控通路的苯中毒免疫相关基因。方法应用含2779条免疫相关基因的cDNA芯片检测7例苯中毒患者和7例正常人的外周血白细胞基因表达谱,观察其在基因表达谱上的差异。进行了基因表达谱聚类分析和比较;筛选有统计学意义的差异表达的免疫相关基因。结果存在差异表达的基因共有38条。在2张以上芯片结果中表达上调的基因10个,主要包括有CD59、TRA@、MCP等。在2张以上芯片结果中表达下调的基因14个,有HLA-DMB、HLA-DQA1、HLA-DPB1、ITGB2、PFC等基因。聚类分析揭示38条基因的基因表达谱特征存在关联。结论通过有统计学意义的差异表达的苯中毒免疫相关基因,推测免疫调控通路在苯中毒发病中可能扮演重要角色。基因芯片技术是分析不同研究对象之间基因差异表达的有效手段。
OBJECTIVE: To search for the genes related to benzene poisoning related to benzene poisoning in the pathogenesis of benzene poisoning through gene expression profiling. Methods The gene expression profiles of peripheral blood leucocytes in 7 benzene poisoning patients and 7 normal controls were detected by cDNA microarray with 2,779 immune related genes and their differences in gene expression profile were observed. The gene expression profiles were clustered and compared. The differentially expressed immune related genes with statistical significance were screened. Results There were 38 differentially expressed genes. 10 genes that were up-regulated in more than two chip results mainly include CD59, TRA @, MCP and so on. There are 14 genes that are down-regulated in more than two chip results, including HLA-DMB, HLA-DQA1, HLA-DPB1, ITGB2 and PFC. Clustering analysis revealed that 38 genes were related to the gene expression profiles. Conclusions There are statistically significant differences in the expression of benzene poisoning immune-related genes, speculated that immune regulation pathway may play an important role in the pathogenesis of benzene poisoning. Gene chip technology is an effective means of analyzing the gene expression differences between different research objects.