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表面增强拉曼光谱(SERS)是一种基于纳米颗粒的拉曼光谱,可以高灵敏度地检测流感病毒等重要病原微生物,鉴定不同毒株间的差异。为了建立一种快速检测流感病毒SERS的方法,本实验利用SERS技术对流感病毒H1N1亚型不同毒株在不同温度和pH值的条件下进行了病毒毒价强弱的检测,将流感病毒样品与金纳米颗粒混合静置后用拉曼共聚焦显微镜进行激光扫描。结果显示在pH为7.2、温度为37℃的条件下3个H1N1亚型的毒株SERS检测结果显示均出现至少1个大于(或等于)3 000的峰值,该状态下病毒毒价最强,最适合病毒生长。另外,细胞生物学方法与SERS技术结果一致,检测中均表现出较好的稳定性和准确性。
Surface-enhanced Raman spectroscopy (SERS) is a nanoparticle-based Raman spectroscopy that can detect important pathogenic microorganisms such as influenza virus with high sensitivity and identify differences between different strains. In order to establish a rapid detection of influenza virus SERS method, this experiment using SERS technology on different strains of influenza virus H1N1 subtypes of viruses at different temperatures and pH conditions for the detection of virulent price, the influenza virus samples and After the gold nanoparticles were mixed, laser scanning was performed with a Raman confocal microscope. The results showed that at pH 7.2 and 37 ℃, the SERS detection results of the three H1N1 subtypes all showed at least one peak greater than (or equal to) 3,000, the strongest virus toxin in this condition, Most suitable for virus growth. In addition, the cell biology method and SERS technology results are consistent, the test showed good stability and accuracy.