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目的探讨糖尿病小鼠微循环功能变化。方法 BALB/c小鼠随机分为对照组(n=10)和糖尿病组(n=20)。常规STZ制备糖尿病模型,心脏灌流后取材,HE染色观察胰腺组织及胰岛微血管形态学及病理改变。用免疫组织化学染色,观察胰岛及胰岛微血管内皮细胞的形态学及病理改变、胰岛素及胰岛微血管PECAM-1的表达。用Moor LDLS检测皮肤微循环血流灌注水平;用Moor VMS-LDF检测胰腺微循环血流灌注及微血管自律运动功能。结果糖尿病小鼠胰岛形态不规则,完整性破坏;胰岛微血管内皮细胞PECAM-1表达失去连续性;胰岛中胰岛素染色阳性面积显著低于对照组(P<0.05)。糖尿病组小鼠皮肤微循环总血流灌注显著降低(P<0.01)。糖尿病组小鼠胰腺微血管自律运动频率和振幅均显著低于对照组(P<0.01,P<0.001)。结论 STZ诱导的BALB/c糖尿病小鼠微循环功能受损。
Objective To investigate the changes of microcirculation in diabetic mice. Methods BALB / c mice were randomly divided into control group (n = 10) and diabetic group (n = 20). The diabetic model was established by routine STZ. After cardiac perfusion, the morphological and pathological changes of pancreas and islet microvessels were observed by HE staining. Immunohistochemical staining was used to observe the morphological and pathological changes of islet and islet microvascular endothelial cells and the expression of PECAM-1 in insulin and islet microvessels. Moor LDLS was used to detect the microcirculation perfusion of the skin. Moor VMS-LDF was used to detect microcirculation perfusion and autonomic motor activity. Results The morphology of islets in diabetic mice was irregular and the integrity was destroyed. The expression of PECAM-1 in islet microvascular endothelial cells lost continuity. The positive area of islet insulin in islets was significantly lower than that in control group (P <0.05). The total microcirculation total blood perfusion in diabetic mice was significantly decreased (P <0.01). The frequency and amplitude of autonomic movement of pancreas in diabetic group were significantly lower than those in control group (P <0.01, P <0.001). Conclusion The microcirculation function of STZ-induced BALB / c diabetic mice is impaired.