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利用已完成基因组测序的两个水稻品种日本晴和 9311 的数据库成功开发出水稻微卫星新标记,并利用由 90 个单株组成的日本晴 9311 F2作图群体,构建了一张包含 152 个 SSR 标记位点、覆盖基因组总长度 2 455.7 cM 的连锁图谱,有 46 个 SSR 新标记为自主开发,该图谱标记间的平均遗传距离为 16.16 cM;并将未能在 Temnykh 等人(2001)构建的图谱上定位的微卫星标记 RM345 和 RM494 定位在第 6 染色体上。通过与 Temnykh 等人(2001)和兰涛等人(2003)所构建的图谱从作图群体的类型和大小、标记的类型和数量、标记在染色体上的线性排列顺序等几个方面进行比较,所绘制的图谱其标记在染色体线性排列上与 Temnykh 等人绘制的图谱具有很高的一致性,达 93.81%。
Using microsatellite markers Nipponbare and 9311, two rice cultivars Nipponbare and Nipponbare 9311, which have been successfully genomic sequenced, were used to develop a new microsatellite marker for rice. Using 90 lines of Nipponbare 9311 F2 mapping population, a DNA fragment containing 152 SSR markers Point and covering 4645.7 cM of the total genome length, 46 SSR markers were newly developed for self-development with an average genetic distance of 16.16 cM between the markers and would not be mapped on the map constructed by Temnykh et al. (2001) Localized microsatellite markers RM345 and RM494 are located on chromosome 6. According to the maps constructed by Temnykh et al. (2001) and Lan Tao et al. (2003), we compare the types and sizes of mapping groups, the type and number of markers, the linear arrangement order of markers on chromosomes, The maps of the markers were highly consistent with the maps drawn by Temnykh et al. In the linear arrangement of chromosomes, reaching 93.81%.