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目的海产品中携带病毒的核酸检测的两种前处理方法的比较。方法以市售的甲肝疫苗作为标准品,梯度稀释成不同浓度的样品,对海产品染毒后,分别采取参照GB/T22287-2008贝类中甲型肝炎病毒检测方法的前处理和使用PBS拍碎海产品消化腺这种样品前处理方法进行标本处理并提取病毒核酸,并采样荧光PCR方法检测,对甲肝病毒核酸进行定性和半定量检测,从而比较两种处理方法的差异性。结果用两种前处理方法均能检测到甲肝病毒。疫苗液、方法 1和方法 2处理的样品,其检出最高稀释倍数的lgCCID50(细胞培养半数感染量)分别为1、2和4。结论海产品的病毒核酸检测可以采用处理方法 1,该方法既简便易操作,灵敏性也优于方法 2。
Comparison of two pre-treatment methods for the detection of nucleic acids carrying the virus in marine products. Methods Commercially available hepatitis A vaccine was used as a standard sample and diluted to different concentrations. Samples of marine products were treated with PBS after being pretreated with reference to GB / T22287-2008 Hepatitis A virus detection method The digestion gland of this sample was treated by sample pretreatment and the viral nucleic acid was extracted and sampled by fluorescent PCR method. The qualitative and semi-quantitative detection of hepatitis A virus nucleic acid was carried out to compare the differences of the two treatment methods. Results Hepatitis A virus was detected by both pretreatment methods. The vaccine solution, samples treated with Method 1 and Method 2 showed the lg CCID50 (half the cell culture inoculum) at the highest dilution of 1, 2 and 4, respectively. Conclusion The detection of viral nucleic acids in marine products can be processed by the method of treatment 1, which is simple and easy to operate and has better sensitivity than method 2.