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目的:探讨宫颈癌组织中人乳头瘤病毒(HPV)16E6 mRNA与survivin蛋白表达的情况及其相互关系。方法:选取宫颈癌患者40例、宫颈上皮瘤变患者90例(Cl N 1、Cl N 2、Cl N 3分别30例)及宫颈炎妇女30例,分别检测上述各类患者宫颈组织中HPV16E6 mRNA与survivin蛋白的表达情况,并进行相关性分析。结果:宫颈癌、宫颈上皮瘤变(Cl N 1、Cl N 2、Cl N 3)患者的HPV16E6 mRNA表达水平显著高于慢性宫颈炎患者,且HPV16E6 mRNA表达水平在宫颈癌>Cl N 3>Cl N 2>Cl N1>慢性宫颈炎患者且组间比较差异均有统计学意义(P<0.05)。不同年龄、临床分期、病理类型的宫颈癌患者宫颈癌组织中的survivin蛋白、HPV16 E6蛋白表达差异无统计学意义(P>0.05),不同病理分化的宫颈癌患者survivin蛋白、HPV16 E6蛋白表达差异有统计学意义(P<0.05),低分化患者的survivin蛋白、HPV16 E6蛋白表达显著高于高分化、中分化患者(P<0.05)。对HPV16E6蛋白及mRNA分别与survivin蛋白进行Spearman相关性,结果显示HPV16E6蛋白与survivin蛋白呈正相关关系(r=0.604,P<0.001),HPV16E6 mRNA与survivin蛋白呈正相关关系(r=0.615,P<0.001)。结论:HPV16E6 mRNA与宫颈病变有一定的关系,HPV16E6 mRNA与survivin蛋白在宫颈癌组织中的表达具有一定的关系。
Objective: To investigate the expression of human papillomavirus (HPV) 16E6 mRNA and survivin protein in cervical cancer and their correlations. Methods: Forty patients with cervical cancer, 90 patients with cervical epithelial neoplasia (Cl N 1, Cl N 2, Cl N 3, 30 patients) and 30 cervicitis women were selected. The expressions of HPV16E6 mRNA And survivin protein expression, and correlation analysis. Results: The expression of HPV16E6 mRNA in patients with cervical cancer and cervical epithelial neoplasia was significantly higher than that in patients with chronic cervicitis, and the expression of HPV16E6 mRNA was higher in cervical cancer than in ClN 3> Cl N 2> Cl N1> patients with chronic cervicitis and the differences between the two groups were statistically significant (P <0.05). There was no significant difference in the expression of survivin protein and HPV16 E6 protein between cervical cancer patients of different ages, clinical stages and pathological types (P> 0.05). The differences of survivin protein and HPV16 E6 protein between different pathological types of cervical cancer patients (P <0.05). The expression of survivin protein and HPV16 E6 in poorly differentiated patients was significantly higher than that in well-differentiated and moderately differentiated patients (P <0.05). The results of Spearman correlation between HPV16E6 protein and survivin protein showed that there was a positive correlation between HPV16E6 protein and survivin protein (r = 0.604, P <0.001), HPV16E6 mRNA and survivin protein (r = 0.615, P <0.001) ). Conclusion: HPV16E6 mRNA and cervical lesions have a certain relationship, HPV16E6 mRNA and survivin protein in cervical cancer has a certain relationship.