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研究目的:比较花椒挥发油与其主要成分作为经皮促透剂的皮肤细胞毒性,对系列不同油水分配系数药物促透作用特征及其作用机制。创新要点:选择系列不同油水分配系数中药活性成分简化并表征中药制剂中复杂有效成分,对比研究花椒挥发油与其主要成分的经皮促透作用特征差异。研究方法:利用表皮角质形成细胞(HaCaT)和真皮成纤维细胞(CCC-ESF-1)测定其皮肤细胞毒性,选择系列不同油水分配系数中药活性成分作为模型药物测定花椒挥发油与其主要成分的经皮促透作用特征差异,并在此基础上采用傅利叶变换红外光谱(FTIR)等研究其促透作用机制。重要结论:花椒挥发油相对于其单一组成成分具有更好的经皮促透效果。
OBJECTIVE: To compare the cytotoxicity of the essential oil of Zanthoxylum bungeanum with its main component as a skin transdermal enhancer, and to characterize the mechanism of drug permeation through different series of oil-water partition coefficients. Innovative points: Select a series of different oil-water partition coefficient of traditional Chinese medicine active ingredients to simplify and characterize the complex active ingredients in traditional Chinese medicine preparation, comparative study of the volatile oil and its main components of percutaneous permeability characteristics differences. Methods: The skin cytotoxicity was determined by using cuticular keratinocytes (HaCaT) and dermal fibroblasts (CCC-ESF-1). A series of traditional Chinese medicine active ingredients with different oil-water partition coefficients were selected as the model drug to determine the percutaneous Permeability characteristics differences, and on this basis using Fourier transform infrared spectroscopy (FTIR) to study its perfused mechanism. Important conclusion: The volatile oil of Zanthoxylum bungeanum has a better transdermal anti-permeability effect than its single component.