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目的建立同时检测鸡肉中睾酮、孕酮、去甲雄三烯醇酮、氟氢甲睾酮、己烯雌酚5种性激素残留量的方法。方法鸡肉经前处理后,用高效液相色谱-串联质谱法(LC-MS/MS)检测5种性激素,内标法定量。结果方法的检出限为0.01～0.06μg/kg;加标回收率为81.9%～112.6%;测定精密度均小于3.0%;睾酮、孕酮、去甲雄三烯醇酮、己烯雌酚在1ng/ml～40 ng/ml范围内、氟氢甲睾酮在2 ng/ml～80 ng/ml范围内具有良好的线性。结论该方法灵敏度高,杂质干扰小,特异性强,是测定鸡肉中性激素残留的理想方法。
Objective To establish a method for the simultaneous determination of five hormones in testosterone, progesterone, norepinephrine, dydrogesterone and diethylstilbestrol in chicken meat. Methods After pretreatment, five sex hormones were detected by high performance liquid chromatography-tandem mass spectrometry (LC-MS / MS) and quantified by internal standard method. Results The detection limits were 0.01 ~ 0.06 μg / kg. The recoveries were 81.9% ~ 112.6%. The precision was less than 3.0%. The testosterone, progesterone, In the range of ~ 40 ng / ml, DHMT has a good linearity in the range of 2 ng / ml ~ 80 ng / ml. Conclusion The method is sensitive, impurity-free and specific. It is an ideal method for the determination of neutral steroid residues in chicken.