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目的研究赤道几内亚比奥科岛(Bioko Island)恶性疟原虫分离株裂殖子表面蛋白1(PfMSP-1)基因和裂殖子表面蛋白2(PfMSP-2)基因分型。方法从比奥科岛采集疟疾患者血样181份,用显微镜法和荧光定量PCR鉴定虫种。采用巢式PCR分别扩增PfMSP-1和PfMSP-2中具有型特异性的片段,进行等位基因分型。结果 PfMSP-1基因分型:181份恶性疟患者血样中有178份扩增出PfMSP-1基因片段(98.34%),其中K1、MAD20和RO33基因型片段分别为171份(94.48%)、175份(96.69%)和126份(69.61%)。混合感染率为97.24%;PfMSP-2基因分型:181份血样中有173份PfMSP-2基因片段(95.58%),其中48份(26.52%)单独扩增到3D7型基因片段,4份(2.21%)单独扩增到FC27型基因片段,混合感染率为66.85%。结论赤道几内亚比奥科岛PfMSP-1和PfMSP-2等位基因型分布范围极广。混合感染是比奥科岛疟疾的主要类型。
Objective To study the genotyping of PfMSP-1 gene and merozoite surface protein 2 (PfMSP-2) of Plasmodium falciparum isolates from Bioko Island in Equatorial Guinea. Methods 181 blood samples of malaria patients were collected from Bioko Island, and the species were identified by microscopy and fluorescence quantitative PCR. Nested PCR was used to amplify the type-specific fragments of PfMSP-1 and PfMSP-2 respectively for allelic typing. Results PfMSP-1 genotyping: PfMSP-1 gene fragment (98.34%) was amplified from 178 blood samples of 181 patients with P. falciparum. The K1, MAD20 and RO33 genotypes were 171 (94.48%), 175 (96.69%) and 126 (69.61%). The mixed infection rate was 97.24%. PfMSP-2 genotyping: 173 PfMSP-2 gene fragments (95.58%) were detected in 181 blood samples, of which 48 (26.52%) were amplified to 3D7 gene fragment separately and 4 2.21%) separately amplified to FC27 gene fragment, the mixed infection rate was 66.85%. Conclusion The distributions of PfMSP-1 and PfMSP-2 alleles in Bioko Island in Equatorial Guinea range widely. Mixed infection is the main type of malaria in Bioko Island.