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1 前言 在生物显微镜观察方面,最先应考虑的必然是不损坏生物本身的活性状态,维持水分、离子浓度、氧和养分的流通,而且在光学观察时,无论是热还是光子能量方面都必须保留在细胞不受损伤的照射量和光能量内。从这种观点出发,用高强度激光照射的非线性光学效应观察细胞,被认
1 Introduction In the field of biological microscopy, the first consideration should be that it does not damage the biological activity itself, maintain the flow of water, ions, oxygen and nutrients, and that in optical observation both the heat and the photon energy must be Retained in the amount of exposure and light energy of the cells are not damaged. From this point of view, observation of cells by the nonlinear optical effect of high-intensity laser irradiation has been considered