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荧光探针技术可以用来测定药物核酸相互作用的强度。通过药物与核酸相互作用,使DNA与探针键合的程度减小,反映在探针荧光光谱的改变,从而可以了解药物和核酸的作用机理。相互作用常数D为DNA+探针中加入药物后探针的荧光强度相对于DNA+探针荧光强度减去纯探针荧光强度的下降百分数。文章利用盐酸小檗碱(Berberine)作为探针,测定几种抗癌药物加入DNA与荧光探针(盐酸小檗碱)混合溶液的荧光谱,探讨它们与DNA的相互作用。并根据相同浓度的不同药物与核酸相互作用的常数D确定作用强弱。
Fluorescent probe technology can be used to determine the strength of drug nucleic acid interactions. The interaction between the drug and the nucleic acid reduces the degree of DNA binding to the probe, which is reflected in the change of the fluorescence spectrum of the probe so that the mechanism of action of the drug and the nucleic acid can be understood. The interaction constant, D, is the percent decrease in fluorescence intensity of the probe relative to the DNA + probe fluorescence intensity minus the pure probe fluorescence intensity after DNA + probe drug addition. In this paper, the fluorescence spectra of several anticancer drugs mixed with DNA and fluorescent probe (berberine hydrochloride) were determined using Berberine as a probe to investigate their interaction with DNA. And according to the same concentration of different drugs and nucleic acid interaction constant D to determine the role of strength.