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目的:观察缺氧复氧诱导人脐静脉内皮细胞凋亡现象及人参皂苷Rb1的干预效应。方法:体外培养人脐静脉内皮细胞,建立内皮细胞缺氧复氧模型,采用TUNEL技术和DNA凝胶电泳观察缺氧不同时间(0、3、6、12、24 h)后复氧对内皮细胞凋亡的影响及Rb1的干预效应。结果:随着缺氧时间延长,复氧后脐静脉内皮细胞凋亡率逐渐升高;Rb1组内皮细胞凋亡率显著低于缺氧复氧组(P<0.05)。结论:内皮细胞凋亡是缺氧复氧损伤的一种重要形式,人参皂苷Rb1通过抑制缺氧复氧诱导内皮细胞凋亡而起到内皮细胞保护效应。
Objective: To observe the apoptosis of human umbilical vein endothelial cells induced by hypoxia and reoxygenation and the intervention effect of ginsenoside Rb1. METHODS: Human umbilical vein endothelial cells were cultured in vitro to establish a model of endothelial cell hypoxia and reoxygenation. TUNEL technique and DNA gel electrophoresis were used to observe the effects of reoxygenation on endothelial cells at different time (0, 3, 6, 12 and 24 h). The effect of apoptosis and the intervention effect of Rb1. Results: With the prolonged hypoxia, the apoptosis rate of umbilical vein endothelial cells gradually increased after reoxygenation; the apoptosis rate of endothelial cells in Rb1 group was significantly lower than that in the hypoxia-reoxygenation group (P<0.05). CONCLUSION: Endothelial cell apoptosis is an important form of hypoxia-reoxygenation injury. Ginsenoside Rb1 plays an endothelial cell protective effect by inhibiting apoptosis of endothelial cells induced by hypoxia-reoxygenation.