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【目的】明确温度对西伯利亚蝗Gomphocerus sibiricus成虫呼吸代谢关键酶活性的影响。【方法】18~42℃范围内,以3℃为梯度,将西伯利亚蝗成虫置于光照培养箱黑暗条件下处理4 h后,利用生化方法测定3-磷酸甘油醛脱氢酶(GAPDH)、3-磷酸甘油脱氢酶(GPDH)、3-羟酰辅酶A脱氢酶(HOAD)、柠檬酸合成酶(CS)和乳酸脱氢酶(LDH)5种呼吸代谢关键酶活性。【结果】随温度升高,西伯利亚蝗成虫体内5种呼吸代谢关键酶活性先增后减,相同温度下雄虫的酶活性均高于雌虫;18℃下雌雄成虫的5种关键代谢酶活性最低,39℃下雌虫的GAPDH和CS活性最高,雄虫CS活性在36℃时最高;不同处理温度下,雌虫之间、雄虫之间关键代谢酶活性差异均显著(P<0.05)。不同处理温度下雌雄虫GAPDH与HOAD活性比值均大于或接近于1.0,说明低温阶段西伯利亚蝗呼吸代谢消耗以糖类为主,高温胁迫下糖类和脂类同时被利用。【结论】温度对西伯利亚蝗成虫体内呼吸代谢关键酶活性有显著影响,高温胁迫下西伯利亚蝗可通过调整呼吸代谢强度和改变消耗底物种类提高自身的适应能力。
【Objective】 The objective of this study was to determine the effect of temperature on the key enzymes of respiratory metabolism in adult Gomphocerus sibiricus. 【Method】 The adults of Siberian locusts were exposed to darkness in a light incubator for 4 h at 18 ℃ to 42 ℃ with a gradient of 3 ℃. The levels of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 3 Five key enzymes in respiratory metabolism were GPDH, HOAD, CS and LDH. 【Result】 The results showed that the activities of five key enzymes in the respiratory metabolism increased firstly and then decreased with the increase of temperature. The activity of five key metabolic enzymes of male and female adults at 18 ℃ was higher than that of females at the same temperature While the activity of GAPDH and CS was the highest at 39 ℃. The CS activity of male was the highest at 36 ℃. The differences of key metabolic enzyme activities between females and males were significant at different treatment temperatures (P <0.05) . The ratio of GAPDH to HOAD activity of both male and female was higher or closer to 1.0 at different treatment temperatures, indicating that the main metabolic traits of Siberian locusts were carbohydrates at low temperature, and sugars and lipids were also used under high temperature stress. 【Conclusion】 Temperature has a significant effect on the key enzyme activity of respiratory metabolism in locusts of Siberian insects. Under high temperature stress, Siberian locusts can improve their adaptability by adjusting the metabolic intensity of respiration and changing the type of substrates consumed.